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KMID : 0545120120220071029
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 7 p.1029 ~ p.1033
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Protein Tyrosine Phosphatase Profiling Analysis of HIB-1B Cells during Brown Adipogenesis
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Choi Hye-Ryung
Kim Won-Kon
Kim Eun-Young
Jung Hye-Yun
Kim Jeong-Hoon
Han Baek-Soo
You Kwan-Hee
Lee Sang-Chul
Bae Kwang-Hee
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Abstract
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A number of evidence have been accumulated that the regulation of reversible tyrosine phosphorylation, which can be regulated by the combinatorial activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), plays crucial roles in various biological processes including differentiation. There are a total of 107 PTP genes in the human genome, collectively referred to as the “PTPome.” In this study, we performed PTP profiling analysis of the HIB-1B cell line, a brown preadipocyte cell line, during brown adipogenesis. Through RT-PCR and real-time PCR, several PTPs showing differential expression pattern during brown adipogenesis were identified. In the case of PTP-RE, it was shown to decrease significantly until 4 days after brown adipogenic differentiation, followed by a dramatic increase at 6 days. The overexpression of PTP-RE led to decreased brown adipogenic differentiation via reducing the tyrosine phosphorylation of the insulin receptor, indicating that PTP-RE functions as a negative regulator at the early stage of brown adipogenesis.
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KEYWORD
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Brown adipogenesis, Protein tyrosine phosphatase, PTPome, PTP-RE
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